H2N2V1, 1957, Analysis, bibRecord, 000055

Hemagglutinin stalk antibodies elicited by the 2009 pandemic influenza virus as a mechanism for the extinction of seasonal H1N1 viruses

Identifieur interne : 000055 ( 1957/Analysis ); précédent : 000054; suivant : 000056

Hemagglutinin stalk antibodies elicited by the 2009 pandemic influenza virus as a mechanism for the extinction of seasonal H1N1 viruses

Auteurs : Natalie Pica ; Rong Hai ; Florian Krammer ; Taia T. Wang ; Jad Maamary ; Dirk Eggink ; Gene S. Tan ; Jens C. Krause ; Thomas Moran ; Cheryl R. Stein ; David Banach ; Jens Wrammert ; Robert B. Belshe ; Adolfo García-Sastre ; Peter Palese

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2012.

RBID : PMC:3289326

Abstract

After the emergence of pandemic influenza viruses in 1957, 1968, and 2009, existing seasonal viruses were observed to be replaced in the human population by the novel pandemic strains. We have previously hypothesized that the replacement of seasonal strains was mediated, in part, by a population-scale boost in antibodies specific for conserved regions of the hemagglutinin stalk and the viral neuraminidase. Numerous recent studies have shown the role of stalk-specific antibodies in neutralization of influenza viruses; the finding that stalk antibodies can effectively neutralize virus alters the existing dogma that influenza virus neutralization is mediated solely by antibodies that react with the globular head of the viral hemagglutinin. The present study explores the possibility that stalk-specific antibodies were boosted by infection with the 2009 H1N1 pandemic virus and that those antibodies could have contributed to the disappearance of existing seasonal H1N1 influenza virus strains. To study stalk-specific antibodies, we have developed chimeric hemagglutinin constructs that enable the measurement of antibodies that bind the hemagglutinin protein and neutralize virus but do not have hemagglutination inhibition activity. Using these chimeric hemagglutinin reagents, we show that infection with the 2009 pandemic H1N1 virus elicited a boost in titer of virus-neutralizing antibodies directed against the hemagglutinin stalk. In addition, we describe assays that can be used to measure influenza virus-neutralizing antibodies that are not detected in the traditional hemagglutination inhibition assay.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3289326

DOI: 10.1073/pnas.1200039109
PubMed: 22308500
PubMed Central: 3289326

Affiliations:

Links to Exploration step

PMC:3289326

Le document en format XML

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 10029;</nlm:aff>
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 30322</nlm:aff>
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 63103</nlm:aff>
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 10029;</nlm:aff>
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<front><div type="abstract" xml:lang="en"><p>After the emergence of pandemic influenza viruses in 1957, 1968, and 2009, existing seasonal viruses were observed to be replaced in the human population by the novel pandemic strains. We have previously hypothesized that the replacement of seasonal strains was mediated, in part, by a population-scale boost in antibodies specific for conserved regions of the hemagglutinin stalk and the viral neuraminidase. Numerous recent studies have shown the role of stalk-specific antibodies in neutralization of influenza viruses; the finding that stalk antibodies can effectively neutralize virus alters the existing dogma that influenza virus neutralization is mediated solely by antibodies that react with the globular head of the viral hemagglutinin. The present study explores the possibility that stalk-specific antibodies were boosted by infection with the 2009 H1N1 pandemic virus and that those antibodies could have contributed to the disappearance of existing seasonal H1N1 influenza virus strains. To study stalk-specific antibodies, we have developed chimeric hemagglutinin constructs that enable the measurement of antibodies that bind the hemagglutinin protein and neutralize virus but do not have hemagglutination inhibition activity. Using these chimeric hemagglutinin reagents, we show that infection with the 2009 pandemic H1N1 virus elicited a boost in titer of virus-neutralizing antibodies directed against the hemagglutinin stalk. In addition, we describe assays that can be used to measure influenza virus-neutralizing antibodies that are not detected in the traditional hemagglutination inhibition assay.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Banach, David" sort="Banach, David" uniqKey="Banach D" first="David" last="Banach">David Banach</name>
<name sortKey="Belshe, Robert B" sort="Belshe, Robert B" uniqKey="Belshe R" first="Robert B." last="Belshe">Robert B. Belshe</name>
<name sortKey="Eggink, Dirk" sort="Eggink, Dirk" uniqKey="Eggink D" first="Dirk" last="Eggink">Dirk Eggink</name>
<name sortKey="Garcia Sastre, Adolfo" sort="Garcia Sastre, Adolfo" uniqKey="Garcia Sastre A" first="Adolfo" last="García-Sastre">Adolfo García-Sastre</name>
<name sortKey="Hai, Rong" sort="Hai, Rong" uniqKey="Hai R" first="Rong" last="Hai">Rong Hai</name>
<name sortKey="Krammer, Florian" sort="Krammer, Florian" uniqKey="Krammer F" first="Florian" last="Krammer">Florian Krammer</name>
<name sortKey="Krause, Jens C" sort="Krause, Jens C" uniqKey="Krause J" first="Jens C." last="Krause">Jens C. Krause</name>
<name sortKey="Maamary, Jad" sort="Maamary, Jad" uniqKey="Maamary J" first="Jad" last="Maamary">Jad Maamary</name>
<name sortKey="Moran, Thomas" sort="Moran, Thomas" uniqKey="Moran T" first="Thomas" last="Moran">Thomas Moran</name>
<name sortKey="Palese, Peter" sort="Palese, Peter" uniqKey="Palese P" first="Peter" last="Palese">Peter Palese</name>
<name sortKey="Pica, Natalie" sort="Pica, Natalie" uniqKey="Pica N" first="Natalie" last="Pica">Natalie Pica</name>
<name sortKey="Stein, Cheryl R" sort="Stein, Cheryl R" uniqKey="Stein C" first="Cheryl R." last="Stein">Cheryl R. Stein</name>
<name sortKey="Tan, Gene S" sort="Tan, Gene S" uniqKey="Tan G" first="Gene S." last="Tan">Gene S. Tan</name>
<name sortKey="Wang, Taia T" sort="Wang, Taia T" uniqKey="Wang T" first="Taia T." last="Wang">Taia T. Wang</name>
<name sortKey="Wrammert, Jens" sort="Wrammert, Jens" uniqKey="Wrammert J" first="Jens" last="Wrammert">Jens Wrammert</name>
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Hemagglutinin stalk antibodies elicited by the 2009 pandemic influenza virus as a mechanism for the extinction of seasonal H1N1 viruses

Hemagglutinin stalk antibodies elicited by the 2009 pandemic influenza virus as a mechanism for the extinction of seasonal H1N1 viruses

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Abstract

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